Open AccessStructure of a 2‐aminoethyl phosphate‐containing O‐specificpolysaccharide of Proteus penneri 8 from a new serogroup O67
Author(s) -
Knirel Yuriy A.,
Zych Krystyna,
Vinogradov Eugeny V.,
Shashkov Alexander S.,
Sidorczyk Zygmunt
Publication year - 2000
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.2000.01061.x
Subject(s) - proteus mirabilis , polysaccharide , galactose , strain (injury) , stereochemistry , chemistry , biochemistry , proteus , antiserum , microbiology and biotechnology , biology , escherichia coli , antibody , anatomy , gene , immunology
An acidic O‐specific polysaccharide was obtained by mild acid degradation of the Proteus penneri 8 lipopolysaccharide and found to contain d ‐glucose, d ‐galacturonic acid, 2‐acetamido‐2‐deoxy‐ d ‐glucose, 2‐acetamido‐2‐deoxy‐ d ‐galactose, 2‐acetamido‐2,6‐dideoxy‐ l ‐galactose ( l ‐FucNAc) and 2‐aminoethyl phosphate ( P Etn) in the ratios 2 : 1 : 1 : 1 : 1 : 1. 1 H and 13 C NMR spectroscopy was applied to the intact and dephosphorylated polysaccharides, and the following structure of the hexasaccharide repeating unit was established: The O‐specific polysaccharide has a unique structure, and, accordingly, we propose for P. penneri 8 a new Proteus O67 serogroup, in which this strain is at present the single representative. The nature of epitopes on LPS of P. penneri 34, P. mirabilis O16, P. mirabilis O23 and P. vulgaris O22, which cross‐react with O‐antiserum against P. penneri 8, is discussed.