Open AccessCapsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus
Author(s) -
Gloaguen Vincent,
Morvan Henri,
Hoffmann Lucien,
Plancke Yves,
Wieruszeski JeanMichel,
Lippens Guy,
Strecker Gerard
Publication year - 1999
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1999.00879.x
Subject(s) - thermophile , polysaccharide , chemistry , hydrolysis , oligosaccharide , acid hydrolysis , mass spectrometry , biochemistry , nuclear magnetic resonance spectroscopy , stereochemistry , chromatography , enzyme
The capsular polysaccharide produced by the thermophilic cyanobacterium Mastigocladus laminosus has been subjected to a specific degradation with lithium in ethylenediamine. The released undecasaccharide attached to one unit of tetrahydroxycyclopentanecarboxylic acid has been characterized by a combination of 2D nuclear magnetic resonance spectroscopy, mass spectrometry, monosaccharidic composition and linkage analyses. From the overlap of the structure of this oligosaccharide with previously identified di‐, tri‐ and pentasaccharides released by mild acid hydrolysis, the capsular polysaccharide was deduced to have a pentadecasaccharide repeating unit with the following structure: