Isolation and characterization of hydrophobic polypeptides in human bile

Polypeptides were isolated from human bile by extraction with chloroform/methanol, followed by reversed‐phase chromatography in methanol/ethylene chloride and gel filtration in chloroform/methanol. Peptides were characterized by SDS/PAGE, sequence analysis and matrix‐assisted laser desorption ionization/time‐of‐flight mass spectrometry. This identified haemoglobin α chain, ATP synthase lipid‐binding protein subunit 9, an N‐terminal fragment of mac25/insulin‐like growth factor‐binding protein 7 and an internal fragment of monocyte differentiation antigen CD14, all not described previously in bile. In addition, α 1 ‐antitrypsin, known in bile from previous work, was also identified. The hydrophobic character of haemoglobin α chain is not apparent from its amino acid sequence, but the other polypeptides all have major hydrophobic segments. These results show that several proteins are removed upon organic solvent extraction used for delipidation during the preparation of samples for proteome analysis. Several of the polypeptides found are unexpectedly present in bile, suggesting that specific excretion mechanisms may be involved.