Open AccessStructural studies of the O‐specific polysaccharide of Hafnia alvei strain PCM 1207 lipopolysaccharide
Author(s) -
Jachymek Wojciech,
Czaja Jolanta,
Niedziela Tomasz,
Lugowski Czeslaw,
Kenne Lennart
Publication year - 1999
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1999.00813.x
Subject(s) - polysaccharide , chemistry , lipopolysaccharide , strain (injury) , hydrolysis , nuclear magnetic resonance spectroscopy , mass spectrometry , acid hydrolysis , chromatography , stereochemistry , biochemistry , biology , anatomy , endocrinology
The structure of the O‐specific side‐chain of the Hafnia alvei strain PCM 1207 lipopolysaccharide (LPS) has been investigated. Methylation analysis, partial acid hydrolysis, matrix‐assisted laser‐desorption ionization time‐of‐flight (MALDI‐TOF) MS, fast atom bombardment (FAB)‐MS/MS and 1 H‐ and 13 C‐NMR spectroscopy were the principal methods used. Glycerol phosphate was identified as a constituent in the polysaccharide and the following structure of a pentasaccharide repeating unit was established: The polysaccharide is partially (≈ 10%) substituted with O‐ acetyl groups. The lipopolysaccharide was also subjected to high resolution magic angle spinning (HR‐MAS) NMR analysis, which showed both the signals of the O‐specific polysaccharide as well as several signals from unsubstituted core oligosaccharides. This confirmed the presence of the described structure in the native LPS.