Open AccessStructure and splice products of the human gene encoding sds22, a putative mitotic regulator of protein phosphatase‐1
Author(s) -
Ceulemans Hugo,
Van Eynde Aleyde,
PérezCallejón Encarna,
Beullens Monique,
Stalmans Willy,
Bollen Mathieu
Publication year - 1999
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1999.00344.x
Subject(s) - exon , polyadenylation , biology , gene , rna splicing , microbiology and biotechnology , genetics , alternative splicing , transcription (linguistics) , exon skipping , messenger rna , rna , philosophy , linguistics
sds22 is a regulatory subunit of protein phosphatase‐1 that is required for the completion of mitosis in yeast. It consists largely of 11 tandem leucine‐rich repeats of 22 residues that are expected to mediate interactions with other polypeptides, including protein phosphatase‐1. In this paper, we report on the structure of the human gene encoding sds22, designated PPP1R7 . This gene (33 kb) comprises 11 exons, but these do not coincide with the sequences encoding the leucine‐rich repeats. Up to six splice variants can be generated by exon skipping and alternative polyadenylation, as revealed by expressed sequence tag database analysis, RT‐PCR and Northern blot analysis. The sds22 transcripts are expected to encode four different polypeptides. sds22α 1 corresponds to the variant cloned previously from human brain [Renouf et al . (1995) FEBS Lett . 375 , 75–78]. Sds22β 1 is truncated within the ninth repeat and has a short and different C‐terminus. Both variants also exist without the sequence corresponding to exon 2, and these are termed sds22α 2 and sds22β 2 . The 5′‐flanking region of PPP1R7 contains two NF‐Y‐binding CCAAT boxes near the transcription start site and potential binding sites for the transcription factors c‐Myb, Ik‐2 and NF‐1, which are conserved in the mouse gene.