Open AccessThe rainbow trout Oncorhynchus mykiss interleukin‐1β gene has a different organization to mammals and undergoes incomplete splicing
Author(s) -
Zou Jun,
Cunningham Charles,
Secombes Christopher J.
Publication year - 1999
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1999.00121.x
Subject(s) - exon , intron , rainbow trout , biology , trout , gene , microbiology and biotechnology , rna splicing , alternative splicing , gene expression , homology (biology) , northern blot , genetics , rna , fishery , fish <actinopterygii>
The rainbow trout interleukin (IL)‐1β gene consists of six exons/five introns, in contrast to mammals which have seven exons/six introns. The missing exon appears to be at the 5′ end of the gene, probably equivalent to exon 1 or 2 of mammals. Fewer and smaller introns make the trout IL‐1β gene only half the size of mammalian IL‐1β genes. Highest homology (> 60% amino acid similarity) is seen between exon 5 of trout and exon 6 of mammals. Reverse transcription–PCR analysis revealed two additional incompletely spliced variants of the trout IL‐1β gene, containing intron 5 or introns 4 and 5. All three transcripts are detectable in gill, kidney, liver and spleen tissue from bacterially challenged fish but only the fully spliced transcript is detectable in blood. Northern blot analysis revealed that the latter transcript is also induced following stimulation of kidney leucocytes with lipopolysaccharide for 4 h. A second inducible transcript is also detected but is larger (≈ 3 kb) than any of the above, suggesting that it could be from a second gene. Southern blot analysis also suggests at least two copies of the IL‐1β gene or genes related to the 3′ end of the IL‐1β sequence, are present in trout.