Open AccessStructure of the O polysaccharide and immunochemical relationships between the lipopolysaccharides of Pseudomonas syringae pathovar tomato and pathovar maculicola
Author(s) -
Knirel Yuriy A.,
Ovod Vladimir V.,
Zdorovenko Galina M.,
Gvozdyak Rostislav I.,
Krohn Kai J.
Publication year - 1998
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1998.2580657.x
Subject(s) - pathovar , pseudomonas syringae , stereochemistry , heteronuclear molecule , chemistry , polysaccharide , nuclear magnetic resonance spectroscopy , rhamnose , biology , pseudomonas , pseudomonadaceae , biochemistry , bacteria , genetics , gene
O polysaccharides (OPS) of the lipopolysaccharides (LPS) of Pseudomonas syringae pathovar tomato GSPB 483 and pathovar maculicola IMV 381 were studied by 1 H‐NMR and 13 C‐NMR spectroscopy, including two‐dimensional COSY, rotating‐frame NOE spectroscopy (ROESY), and H‐detected 1 H, 13 C heteronuclear multiple‐quantum coherence (HMQC) experiments. The OPS from both strains were shown to be chemically identical. Two structurally different types of repeating units (O repeats 1 and 2 ) were elucidated in each OPS. The minor O repeat is a pentasaccharide having the structure 2 . The same structure has been reported earlier for some other OPS of P. syringae . The major O repeat is a hexasaccharide with the novel structure 1 which is distinguished from 2 by the presence of the second lateral residue of 3‐acetamido‐3,6‐dideoxy‐ D ‐galactose ( D ‐Fuc3NAc) and by a different position of substitution of one of the L ‐rhamnose (Rha) residues in the backbone. α‐ D ‐Fuc p 3NAcα‐ D ‐Fuc p 3NAc11↓↓33←2)‐α‐ L ‐Rha p ‐(1←2)‐α‐ L ‐Rha p ‐(1←3)‐α‐ L ‐Rha p ‐(1←3)‐α‐ L ‐Rha p ‐(1← 1 α‐ D ‐Fuc p 3NAc1↓3←2)‐α‐ L ‐Rha p ‐(1←3)‐α‐ L ‐Rha p ‐(1←3)‐α‐ L ‐Rha p ‐(1←3)‐α‐ L ‐Rha p ‐(1← 2 Structural and serological diversity of OPS of strains belonging to P. syringae pv. tomato and pv. maculicola and phylogenetic relatedness of the strains are discussed.