Open AccessStructural studies of the O‐antigenic polysaccharide from Escherichia coli O139
Author(s) -
Marie Christèle,
Weintraub Andrej,
Widmalm Göran
Publication year - 1998
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1998.2540378.x
Subject(s) - heteronuclear molecule , polysaccharide , escherichia coli , two dimensional nuclear magnetic resonance spectroscopy , rhamnose , chemistry , nuclear magnetic resonance spectroscopy , heteronuclear single quantum coherence spectroscopy , sugar , methylation , biochemistry , stereochemistry , microbiology and biotechnology , biology , gene
The structure of the O‐antigenic polysaccharide from Escherichia coli O139 has been investigated with the aid of NMR spectroscopy, and sugar and methylation analyses. The sequence of the sugar residues was determined by NOESY and heteronuclear‐mutiple‐bond‐connectivity NMR experiments. The polysaccharide is composed of heptasaccharide repeating units containing 0.6 mol O ‐acetyl groups/repeating unit. The following structure is concluded, where Rha represents rhamnose.←3)‐α‐ D ‐Glc p NAc‐(1←3)‐α‐ L ‐Rha p ‐(1←4)‐α‐ D ‐Gal p A‐(1←2)‐α‐ L ‐Rha p ‐(1←3)‐α‐ L ‐Rha p ‐(1←2)‐α‐ L ‐Rha p ‐(1←3↑1β‐ D ‐Glc p