Open AccessStructural determination of the O‐antigenic polysaccharide from Escherichia coli O141
Author(s) -
Färnbäck Magnus,
Weintraub Andrej,
Widmalm Göran
Publication year - 1998
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1998.2540168.x
Subject(s) - heteronuclear molecule , polysaccharide , escherichia coli , two dimensional nuclear magnetic resonance spectroscopy , chemistry , nuclear magnetic resonance spectroscopy , stereochemistry , sugar , heteronuclear single quantum coherence spectroscopy , biochemistry , gene
The structure of the O‐antigenic polysaccharide from Escherichia coli O141 has been determined. NMR spectroscopy and sugar and methylation analyses were the principal methods used. The sequence of the sugar residues could be determined by NOESY and heteronuclear multiple‐bond connectivity (HMBC‐) NMR experiments. The polysaccharide is composed of pentasaccharide repeating units with 1 O ‐acetyl group/repeating unit. The following structure, where Rha is 6‐deoxymannose is concluded :α‐ L ‐Rha p 1↓3←3)‐β‐ D ‐Glc p NAc‐(1←4)‐α‐ D ‐Man p ‐(1←3)‐α‐ D ‐Man p 6Ac‐(1←2↑1β‐ D ‐Glc p A