Open AccessStructural characterisation of N‐linked and O‐linked oligosaccharides derived from interferon‐α2b and interferon‐α14c produced by Sendai‐virus‐induced human peripheral blood leukocytes
Author(s) -
Nyman Tuula A.,
Kalkkinen Nisse,
Tölö Hannele,
Helin Jari
Publication year - 1998
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1998.2530485.x
Subject(s) - sendai virus , interferon , glycan , chemistry , peripheral blood , microbiology and biotechnology , fucose , virus , virology , glycoprotein , biology , biochemistry , immunology
We have previously isolated and partially characterised the components of a highly purified interferon‐α (IFN‐α) preparation produced by Sendai‐virus‐induced human peripheral blood leukocytes. Nine IFN‐α species were identified, and two of these were found to be glycosylated [Nyman, T. A., Tölö, H., Parkkinen, J. & Kalkkinen, N. (1998) Identification of nine interferon‐α subtypes produced by Sendai‐virus‐induced human peripheral blood leukocytes, Biochem. J. 329 , 295−302]. Here, we isolated the N‐linked oligosaccharides of IFN‐α14c and the O‐linked chains of IFN‐α2b, and the glycans were characterised by electrospray tandem mass spectrometry and by specific glycosidase digestions monitored by matrix‐assisted laser desorption ionisation time of flight mass spectrometry. The IFN‐α14c N‐glycans were shown to exhibit core‐fucosylated biantennary glycans, with about 10 % carrying an additional α1,3‐linked fucose unit at the antennae. The IFN‐α2b was shown to carry about 50 % core type‐1 disialyltetrasaccharides, 30 % core type‐1 monosialyltrisaccharides and 20 % core type‐2 monosialylpentasaccharides.