Open AccessNon‐detergent sulphobetaines enhance the recovery of membrane and/or cytoskeleton‐associated proteins and active proteases from erythrocytes infected by Plasmodium falciparum
Author(s) -
Blisnick Thierry,
MoralesBetoulle Maria Eugenia,
Vuillard Laurent,
Rabilloud Thierry,
Braun Breton Catherine
Publication year - 1998
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1998.2520537.x
Subject(s) - proteases , plasmodium falciparum , cytoskeleton , microbiology and biotechnology , erythrocyte membrane , membrane protein , chemistry , membrane , biology , biochemistry , malaria , immunology , enzyme , cell
A better understanding of the causative agent's biology and the definition of new targets for the development of drugs and/or specific immune responses is necessary to face the spred of drug‐resistant malaria in developing countries and the absence of an efficient vaccine against this most important infectious disease. Non‐detergent sulphobetaines enhance the recovery and isoelectric focussing of active Plasmodium falciparum proteases, cytoskeleton‐associated proteins and Maurer's cleft‐associated proteins. This is a significant advantage for the purification of such proteins and might help pinpoint their role for red blood cell rupture and merozoite release.