Open AccessImmunochemical studies of the lipopolysaccharide O‐specific polysaccharide of Hafnia alvei PCM 1199 related to H. alvei PCM 1205
Author(s) -
Katzenellenbogen Ewa,
Kocharova Nina A.,
Zatonsky Georgy V.,
Mieszala Malgorzata,
Gamian Andrzej,
Bogulska Maria,
Shashkov Aleksander S.,
Romanowska Elzbieta,
Knirel Yuriy A.
Publication year - 1998
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1327.1998.2510980.x
Subject(s) - chemistry , residue (chemistry) , lipopolysaccharide , hafnia , polysaccharide , hydrolysis , stereochemistry , glycosidic bond , dephosphorylation , acid hydrolysis , biochemistry , organic chemistry , biology , enzyme , cubic zirconia , ceramic , phosphatase , endocrinology
On the basis of chemical analyses and NMR spectroscopic studies, it was found that the O‐specific polysaccharide (O‐PS) isolated from the Hafnia alvei PCM 1199 lipopolysaccharide (LPS) is a glycerol teichoic‐acid‐like polymer having a repeating unit of the following structure : 3)‐β‐ D ‐Qui p 4NAc‐(1←1)‐Gro3 P ‐(O←3)‐β‐ D ‐Gal p ‐(1←3)‐α‐ D ‐Glc p NAc‐(1←26↑∣1 OAcβ‐ D ‐Glc p NAc6∣OAcwhere Qui4NAc is 4‐acetamido‐4,6‐dideoxyglucose and O‐acetylation at both positions is non‐stoichiometric. The glycosidic linkage of the lateral β‐ D ‐Glc p NAc residue is acid‐labile and cleaved from the OPS during mild acid hydrolysis or dephosphorylation with 48 % hydrofluoric acid. Comparative analysis revealed that the structure of the H. alvei PCM 1199 O‐PS is similar to that of H. alvei PCM 1205, which differs in the presence of an additional lateral α‐ D ‐Glc p residue and the position of one of the O ‐acetyl groups only. Accordingly, serological tests revealed a high degree of serological similarity between LPSs and O‐PSs of the two H. alvei strains.