Open AccessCloning, characterization and expression analysis of interleukin‐10 from the common carp, Cyprinus carpio L.
Author(s) -
Savan Ram,
Igawa Daisuke,
Sakai Masahiro
Publication year - 2003
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1033.2003.03854.x
Subject(s) - biology , carp , common carp , microbiology and biotechnology , complementary dna , untranslated region , intron , exon , open reading frame , gene , sequence analysis , peptide sequence , grass carp , cyprinus , genetics , messenger rna , fishery , fish <actinopterygii>
Interleukin (IL)‐10 was cloned from the common carp ( Cyprinus carpio L.) using IL‐10 primers from carp head kidney following stimulation with concanavalin A and lipopolysaccharide. The cDNA consisted of a 1096 bp sequence containing a 55 bp 5′ untranslated region and a 498 bp 3′ untranslated region. An open reading frame of 543 bp encoded a putative 180 amino acid protein with a putative signal peptide of 22 amino acids. The signature motif of IL‐10 is conserved in carp sequence. A 2083 bp genomic sequence of carp IL‐10 was found to contain five exons interrupted by four introns. With the exception of much more compact introns, the genomic structure was similar to that of mammalian IL‐10. By homology, phylogeny and genomic analyses, the carp gene cloned was designated as IL‐10. Carp IL‐10 was expressed in head, kidney, liver, spleen and intestine during the resting phase. The gene was also expressed in head kidney and liver following in vitro stimulation with lipopolysaccharide.