Open AccessAnalysis of flux estimates based on 13 C‐labelling experiments
Author(s) -
Christensen Bjarke,
Karoly Gombert Andreas,
Nielsen Jens
Publication year - 2002
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1033.2002.02959.x
Subject(s) - isotopomers , pentose phosphate pathway , flux (metallurgy) , metabolic flux analysis , labelling , reproducibility , saccharomyces cerevisiae , metabolism , metabolite , metabolic pathway , tricarboxylic acid , chemistry , metabolic network , citric acid cycle , biochemistry , yeast , chromatography , glycolysis , organic chemistry , molecule
Modelling of the fluxes in central metabolism can be performed by combining labelling experiments with metabolite balancing. Using this approach, multiple samples from a cultivation of Saccharomyces cerevisiae in metabolic and isotopic steady state were analysed, and the metabolic fluxes in central metabolism were estimated. In the various samples, the estimates of the central metabolic pathways, the tricarboxylic acid cycle, the oxidative pentose phosphate pathway and the anaplerotic pathway, showed an unprecedented reproducibility. The high reproducibility was obtained with fractional labellings of individual carbon atoms as the calculational base, illustrating that the more complex modelling using isotopomers is not necessarily superior with respect to reproducibility of the flux estimates. Based on these results some general difficulties in flux estimation are discussed.