Open AccessStructural determination of the O‐chain polysaccharide from Agrobacterium tumefaciens , strain DSM 30205
Author(s) -
De Castro Cristina,
De Castro Olga,
Molinaro Antonio,
Parrilli Michelangelo
Publication year - 2002
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1033.2002.02955.x
Subject(s) - agrobacterium tumefaciens , bacteria , strain (injury) , agrobacterium , polysaccharide , cell wall , biology , microbiology and biotechnology , rhizobiaceae , transgene , genetically modified crops , chemistry , botany , biochemistry , gene , genetics , anatomy , symbiosis
Agrobacterium tumefaciens is a Gram‐negative, phytopathogenic bacterium and is characterized by an unique mode of action on dicotyledonous plants: it is able to genetically modify the host, and because of this feature, it is used as a tool for transgenic plants. Many experiments have demonstrated that lipopolysaccharides (LPSs) play an important role for the disease development, as they are involved in the adhesion process of the bacterium on the plant cell wall. Despite the wealth of information on the role of LPS on phytopathogenesis, the present paper appears as the first report on the molecular primary structure of the O‐chain produced from Agrobacterium . Its repeating unit was determined by means of chemical and spectroscopical analysis, and has the following structure: (3)‐α‐ d ‐Ara f ‐(1→3)‐α‐ l ‐Fuc p ‐(1→.