Open AccessStructure of the O‐polysaccharide and classification of Proteus mirabilis strain G1 in Proteus serogroup O3
Author(s) -
Sidorczyk Zygmunt,
Zych Krystyna,
Toukach Filip V.,
Arbatsky Nikolay P.,
Shashkov Alexander S.,
Knirel Yuriy A.
Publication year - 2002
Publication title -
european journal of biochemistry
Language(s) - English
Resource type - Journals
eISSN - 1432-1033
pISSN - 0014-2956
DOI - 10.1046/j.1432-1033.2002.02782.x
Subject(s) - proteus mirabilis , polysaccharide , strain (injury) , lipopolysaccharide , proteus , microbiology and biotechnology , chemistry , heteronuclear molecule , biology , stereochemistry , nuclear magnetic resonance spectroscopy , escherichia coli , biochemistry , gene , immunology , anatomy
The O‐chain polysaccharide of the lipopolysaccharide (LPS) of a previously nonclassified strain of Proteus mirabilis termed G1 was studied by sugar analysis and 1 H and 13 C NMR spectroscopy, including 2D COSY, TOCSY, rotating‐frame NOE (ROESY), H‐detected 1 H, 13 C HMQC, and heteronuclear multiple‐bond correlation (HMBC) experiments. The following structure of the polysaccharide was established: where d ‐GalA6( l ‐Lys) stands for N α ‐( d ‐galacturonoyl)‐ l ‐lysine. The structure of the O‐polysaccharide of P. mirabilis G1 is similar, but not identical, to that of P. mirabilis S1959 and OXK belonging to serogroup O3. Immunochemical studies with P. mirabilis G1 and S1959 anti‐(O‐polysaccharide) sera revealed close LPS‐based serological relatedness of P. mirabilis G1 and S1959, and therefore it was suggested to classify P. mirabilis G1 in serogroup O3 as a subgroup. P. mirabilis G1 and S1959 anti‐(O‐polysaccharide) sera also cross‐reacted with LPS of␣ P. mirabilis strains from two other serogroups containing␣ d ‐GalA6( l ‐Lys) in the O‐polysaccharide or in the core region.