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Circulation and distribution of autotransfused fresh, liquid‐preserved and cryopreserved baboon platelets
Author(s) -
Valeri C. R.,
Giorgio A.,
Macgregor H.,
Ragno G.
Publication year - 2002
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2002.00229.x
Subject(s) - platelet , cryopreservation , baboon , chemistry , andrology , medicine , biology , embryo , microbiology and biotechnology
Background and Objectives Studies were carried out in five healthy male baboons to determine the 111 indium oxine ( 111 In‐oxine) survival of autologous fresh, liquid‐preserved and cryopreserved platelets. Simultaneous organ‐distribution studies were performed to determine the percentage uptake of platelets by the spleen and/or liver. Materials and Methods Each of five baboons was transfused, on three different occasions, with autologous fresh platelets stored at 22 °C for 18 h, liquid‐preserved platelets stored at 22 °C for 5 days and washed previously frozen platelets, labelled with 111 In‐oxine. Results In vivo recovery at 2 h was 81% for the fresh platelets, 54% for the previously frozen platelets and 44% for the 5‐day‐old liquid‐preserved platelets. The weighted mean life span was 5·4 days for fresh platelets, 4·2 days for previously frozen platelets and 2 days for liquid preserved platelets. Increased radioactivity was detected over the liver 2 h after transfusion for both the previously frozen and liquid‐preserved platelets. Conclusions Cryopreserved platelets and liquid‐preserved platelets stored at 22 °C for 5 days had reduced survival 2 h post‐transfusion and reduced life span values compared to fresh platelets. In addition, the finding of increased radioactivity over the liver in the baboons that received cryopreserved and liquid‐preserved platelets suggested that the liver was the site for removal of the non‐viable platelets.