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A novel true volumetric method for the determination of residual leucocytes in blood components
Author(s) -
Cassens U.,
Greve B.,
Taper K.,
Nave B.,
Severin E.,
Sibrowski W.,
Göhde W.
Publication year - 2002
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2002.00176.x
Subject(s) - white blood cell , chromatography , detection limit , blood cell , red blood cell , platelet , residual , medicine , chemistry , immunology , mathematics , algorithm
Background and Objectives Accurate determination of residual leucocytes [white blood cells (WBC)] in blood components is of high clinical importance. To date, several labour‐intensive, time‐consuming or expensive techniques have been used for this purpose. Materials and Methods A method for the determination of residual WBC is described using a novel low‐cost flow‐cytometric cell counter and analyser (CCA). The DNA in WBC was stained using 4′‐6‐diamidino‐2‐phenylindole (DAPI) and WBC were automatically analysed by true volumetric counting of 200‐µl samples (prepared from a 20‐µl undiluted sample). Results Dilution experiments over a range of 0·5–50 WBC/µl showed a linearity of r = 0·998. The detection limit of this method was 0·83 WBC/µl of red blood cell concentrate (RCC) and 0·67 WBC/µl of platelet concentrate (PC), with an accuracy of 95·5%. Conclusion Residual WBC (< 1 WBC/µl) can be accurately counted using the CCA within 2 min and at a total cost of less than € 1 per sample.