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Back‐priming of the RCM1™ leucocyte‐reduction filter: consequences for filtration efficacy
Author(s) -
KjeldsenKragh J.,
Golebiowska E.
Publication year - 2002
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2002.00154.x
Subject(s) - priming (agriculture) , filtration (mathematics) , filter (signal processing) , immunology , medicine , biology , mathematics , computer science , statistics , germination , computer vision , botany
Background and Objectives The RCM1™ leucocyte‐reduction filter has been incorporated into two types of blood‐donation sets of which one uses back‐priming of the leucocyte filter with SAG‐M solution. In this study we have compared the leucocyte‐reduction efficacy of the RCM1™ filter with or without back priming with SAG‐M solution. Materials and Methods This study was a retrospective analysis of quality control data of 3529 leucocyte‐filtered red cell units. Of these, 1002 whole‐blood units were collected in a donation set that was back‐primed by letting the SAG‐M solution in the final storage bag run backwards through the filter into the bag of concentrated red cells. The remaining 2527 units were collected in a donation set that required no back‐priming. Postfiltration leucocyte concentration was assessed using flow cytometry. Results There was a significant trend towards lower leucocyte concentration in the units in which back‐priming of the filter preceded filtration, as compared to units filtered without back‐priming [χ 2 trend = 18·8, degrees of freedom (d.f.) = 1, P < 0·0005]. Conclusions Back‐priming of the RCM1™ filter for red cells with SAG‐M solution is superior to no back‐priming with regard to leucocyte‐reduction efficacy.