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Establishment of the first World Health Organization International Standard for human parvovirus B19 DNA nucleic acid amplification techniques
Author(s) -
Saldanha J.,
Lelie N.,
Yu M. W.,
Heath A.
Publication year - 2002
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2002.00132.x
Subject(s) - nat , nucleic acid , dna , titer , parvovirus , microbiology and biotechnology , chemistry , chromatography , biology , virology , virus , biochemistry , mathematics , statistics
Background and Objectives A collaborative study, involving 26 laboratories from 14 countries, was carried out in order to establish a World Health Organization (WHO) International Standard for human parvovirus B19 (B19) DNA nucleic acid amplification techniques (NAT). Materials and methods Four samples: AA, BB (which were lyophilized), CC and DD (which were liquid preparations) were analysed using several different NAT assays. The mean B19 DNA content of each sample was determined for each laboratory using an end‐point dilution method. Results There was good agreement between the overall mean ‘equivalents’/ml obtained by the different assays. The mean log 10 ‘equivalents’/ml were 5·76 for sample AA, 5·73 for sample BB, 5·82 for sample CC and 7·70 for sample DD. The differences in titre among samples AA, BB and CC were not statistically significant, but the titre of DD was significantly higher. Conclusions Despite the range of NAT assays used in the study, it was possible to calculate the mean B19 DNA concentrations in the four preparations. Lyophilized preparation AA was established as the first International Standard for B19 DNA NAT assays and was assigned a concentration of 10 6 international units (IU)/ml.