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Lymphocyte collection for donor leucocyte infusion from normal donors: estimation of the minimum processed blood volume and safety of the procedure
Author(s) -
Sato H.,
Shiobara S.,
Yasue S.,
Chuhjo T.,
Nakao S.
Publication year - 2001
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2001.00091.x
Subject(s) - apheresis , leukapheresis , medicine , blood volume , adverse effect , plateletpheresis , mean platelet volume , cd3 , platelet , peripheral blood , surgery , anesthesia , urology , immunology , antigen , cd8 , biology , stem cell , cd34 , genetics
Background and Objectives To estimate the minimum volume of processed blood necessary for the purpose of donor leucocyte infusion (DLI), we determined the number of CD3 + cells harvested by apheresis from normal donors and examined adverse events during the procedure. Materials and Methods Leukapheresis utilizing the COBE Spectra‘ was performed a total of 24 times from 12 normal donors. Blood counts were obtained and the number of CD3 + cells was evaluated before and after apheresis and in the peripheral blood products. Complications associated with the procedure were documented. Results Blood products contained, on average, 51·1% CD3 + cells. A linear correlation was found between the number of CD3 + cells collected and the processed blood volume, up to 12 000 ml ( r = 0·930, P < 0·001). Cytoreduction was observed in all donors after apheresis. In particular, the platelet and lymphocyte values decreased to ≈ 70% of the preapheresis value. Two donors reported adverse effects: one was a mild vaso‐vagal reaction and the other was citrate‐related lip paresthesia. These symptoms were mild and disappeared spontaneously. Conclusions We found a linear correlation between the number of CD3 + cells collected and the processed blood volume, which enabled us to estimate the minimum volume of processed blood necessary for DLI. The adverse events observed were acceptable.