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Fcγ receptor‐mediated immune phagocytosis depends on the class of FcγR and on the immunoglobulin‐coated target cell
Author(s) -
Flesch B. K.,
Vöge K.,
Henrichs T.,
Neppert J.
Publication year - 2001
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2001.00081.x
Subject(s) - phagocytosis , antibody , fc receptor , polyclonal antibodies , receptor , immune system , biology , immunoglobulin g , monoclonal antibody , immunology , effector , microbiology and biotechnology , biochemistry
Background and Objectives Three human Fcγ receptors (FcγR) are known to mediate immune phagocytosis. A variety of different phagocytic assays have been described, but their comparability is complicated by the use of different effector cells and different antibody‐coated target cells. The aim of this study was to determine the influence of these variable components on the FcγR‐mediated phagocytosis. Materials and Methods We sensitized human red blood cells (RBC) with polyclonal human anti‐D (huaD), or with human monoclonal anti‐D of the isotypes IgG1 (huIgG1) or IgG3 (huIgG3). Sheep RBC coated with rabbit immunoglobulin (RBC‐RAS) were also used. Monocytes or polymorphonuclear neutrophils (PMN) were incubated with different FcγR‐specific antibodies or their F(ab′) 2 fragments to determine the contribution of the different FcγRs on these effector cells in the phagocytic process of different antibody‐coated target cells. Results huaD‐RBC and huIgG1‐RBC were preferentially ingested via the FcγRI on monocytes and, to a minor extent, also by the FcγRII. PMN ingested these target cells only after induction of the FcγRI by interferon‐γ (IFN‐γ). huIgG3‐RBC extensively formed rosettes with monocytes but were seldom ingested. RAS‐RBC phagocytosis was induced primarily via the FcγRI on monocytes and was mediated by the FcγRII on PMN. Conclusion When performing phagocytosis assays with different effector and target cells, one has to take into account that phagocytosis is mediated by different FcγR, making comparability of these assays more difficult.