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A multicentre evaluation of a new filtration protocol for leucocyte depletion of high‐haematocrit red blood cells collected by an automated blood collection system
Author(s) -
Soli M.,
Blanco L.,
Riggert J.,
MartínezClavel A.,
Lucas C.,
Lunghi M.,
Belloni M.,
Wolf C.,
Van Waeg G.,
Antoon M.
Publication year - 2001
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2001.00078.x
Subject(s) - blood collection , protocol (science) , filtration (mathematics) , medicine , blood preservation , red blood cell , immunology , andrology , emergency medicine , pathology , mathematics , statistics , alternative medicine
Background and Objectives A multicentre trial was set up to evaluate the performance of a new leucodepletion protocol. Materials and Methods Filtration at high haematocrit was started during collection of red blood cell (RBC) products by apheresis with Trima. SAG‐M was added after filtration through the filter. Haematocrits and haemoglobin of the filtered RBCs were measured. Residual leucocytes were determined by Nageotte counting. Results One‐hundred and forty seven procedures were carried out. The haematocrit and haemoglobin contents were 57·3 ± 3·0% and 55·1 ± 4·3 g/unit, respectively. All products showed low residual leucocyte levels (≤ 0·75 × 10 6 /unit; 99·31% < 1 × 10 6 ). Conclusion Immediate, on‐line, high‐haematocrit filtration of red cells collected on Trima resulted in leucoreduced RBCs, which met the AABB and Council of Europe criteria.