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Four Examples of Anti‐TSEN and Three of TSEN‐Positive Erythrocytes
Author(s) -
Story Jill R.,
Lindsay Gwenn,
Rolih Susan,
Co Asuncion,
Rodberg Karen,
Harris Teresa,
Reid Marion E.
Publication year - 2000
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2000.7930175.x
Subject(s) - glycophorin , chemistry , antibody , red blood cell , epitope , antigen , band 3 , monoclonal antibody , microbiology and biotechnology , membrane , immunology , erythrocyte membrane , biochemistry , biology
Background:TSEN (MNS33) is a low‐incidence antigen in the MNS blood group system encoded by hybrid glycophorin genes. TSEN is expressed by a unique amino acid sequence that results from the junction of GPA 58 to GPB 27 , if the GPB carries S antigen. Until this study, only one example of anti‐TSEN had been found. Antibody screening red blood cells (RBCs) positive for both S and s (ref. No. C873) reacted with four patient sera. Initially, the RBCs had been typed as S+s+, but later were typed as S‐s+ in another laboratory. Two other RBC samples, one from a volunteer blood donor (D.L.), the other from a patient whose serum contained anti‐En a FR (J.S.), also gave anomalous results when tested with anti‐S. We sus pected the presence of TSEN‐positive hybrids on all three RBC samples. Materials and Methods: Reactive sera (O.B., E.C., S.K., R.F.) were tested against RBCs with normal MNS phenotypes and with TSEN‐positive RBCs. The RBCs of D.L., J.S. and C873 were tested with anti‐S whose reactivity with S+s+ TSEN+RBCs had been established previously, and with the original example of anti‐TSEN. Immunoblotting was performed on the C873, D.L. and J.S. RBC membranes using a monoclonal antibody to an epitope common to both glycophorin A and glycophorin B. Results: The sera from O.B., E.C., S.K. and R.F. were strongly reactive on the indirect antiglobulin test with TSEN+ RBCs. The RBCs of C873, D.L. and J.S. were typed as TSEN+. Immunoblotting pattern of D.L. and C873 were consistent with TSEN heterozygotes, while that of J.S. was consistent with a TSEN homozygote. Conclusions: Based on the estimated number of screening events with C873 RBCs, the incidence of anti‐TSEN is approximately 1 in 20,000 sera. The antibody is found in patients with and without documented exposure to allogeneic RBCs. All known examples of anti‐TSEN are lgG, but their clinical significance is not known.