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An Experiment with Glycerol‐Frozen Red Blood Cells Stored at ‐80°C for up to 37 years
Author(s) -
Valeri C.R.,
Ragno G.,
Pivacek L.E.,
Cassidy G.P.,
Srey R.,
HanssonWicher M.,
Leavy M.E.
Publication year - 2000
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2000.7930168.x
Subject(s) - glycerol , hemolysis , blood preservation , fresh frozen plasma , red cell , red blood cell , chemistry , frozen section procedure , andrology , biochemistry , medicine , surgery , immunology , platelet
Background and Objectives:Red cells frozen using 40% W/V glycerol are currently FDA approved for frozen storage at ‐80°C for up to 10 years. Materials and Methods: Red cells frozen with 40% W/V glycerol and stored at ‐80°C for up to 37 years were thawed, deglycerolized, and stored at 4°C for 24 h. Results: Red cells frozen for up to 37 years had mean freeze‐thaw‐wash recovery values of 75%, less than 1% hemolysis, and normal ATP, 2,3‐DPG and P50 levels, and 60% of normal RBC K+ levels. Conclusions: Red cells frozen with 40% W/V glycerol can be stored at ‐80°C for up to 37 years with acceptable in vitro results.