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HLA‐C Expression on Platelets: Studies with an HLA‐Cw1‐Specific Human Monoclonal Antibody
Author(s) -
Datema Gert,
Stein Sophia,
Eijsink Chantal,
Mulder Arend,
Claas Frans H.J.,
Doxiadis Ilias I.N.
Publication year - 2000
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2000.7920108.x
Subject(s) - platelet , flow cytometry , monoclonal antibody , human leukocyte antigen , cytotoxicity , immunology , antibody , biology , microbiology and biotechnology , antigen , monoclonal , whole blood , genetics , in vitro
Background and Objectives:The expression of HLA‐C on the surface of platelets is rarely studied due to the lack of proper alloantisera. We addressed this question using an IgM human monoclonal antibody directed against HLA‐Cw1 (VP6G3). Material and Methods: Both flow cytometry and complement dependent cytotoxicity studies were used in the current analysis. Results: The expression of the HLA‐Cw1 antigen on platelets is lower than on peripheral blood lymphocytes as shown by flow cytometry. Variation in expression levels between individuals is also observed. Using this antibody in a complementdependent cytotoxicity assay, we did not observe lysis using platelets as targets, whereas peripheral blood lymphocytes of the same blood donors were adequately lysed. Conclusions: These results confirm that platelets indeed express HLA‐C. Furthermore, the results support the insignificant role of HLA‐C in immunological platelet refractoriness.