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Low Leukocyte Contamination without Filtration by Preparation of Platelet Concentrates in Cylindrical Bags with the Buffy‐Coat Method
Author(s) -
Kostelijk E.H.,
Loos J.A.,
Veldman H.A.,
Korte D.
Publication year - 2000
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2000.7920087.x
Subject(s) - buffy coat , platelet , contamination , centrifugation , filtration (mathematics) , platelet concentrate , yield (engineering) , chemistry , hematocrit , chromatography , materials science , surgery , medicine , immunology , composite material , mathematics , biology , ecology , statistics
Background and Objectives:The buffy‐coat (BC) method for platelet concentrate (PC) preparation was modified in order to obtain leukodepleted PCs from single BCs without filtration. Materials and Methods: BCs were centrifuged in cylindrical BC bags and the optimal centrifugation conditions and optimal hematocrit were determined. Results: With optimal conditions, a tenfold lower leukocyte contamination was obtained compared with the conventionally shaped, wide BC bag (0.3 ± 0.19 versus 3.0 ± 1.71 × 10 6 leukocytes per unit; 85‐ml BCs). The platelet yield obtained with the cylindrical bag did not differ significantly from the yield obtained with the conventional bag (56 ± 16.4 versus 61 ± 15 × 10 9 platelets per PC). Furthermore, when PCs were prepared from 100‐ml BCs in cylindrical bags, a leukocyte contamination of 0.2 ± 0.11 × 10 6 and a platelet content of 6 ± 13.5 × 10 9 per PC were obtained. Conclusion: The use of cylindrical BC bags reduced the leukocyte contamination in PCs to a level required for leuko‐depletion without affecting platelet recovery.