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Collection Efficiencies of CD34+ Progenitor Cells and Mononuclear Cells in Leukapheresis Products Quantified by Flow Cytometry and Calculated on the Basis of a New Formula
Author(s) -
Schlenke Peter,
Frohn Christoph,
Hennig Holger,
Weber Klaus,
Kisro Jens,
Saballus Martin,
Kirchner Holger,
Klüter Harald
Publication year - 2000
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2000.7840242.x
Subject(s) - leukapheresis , peripheral blood mononuclear cell , cd34 , apheresis , progenitor cell , flow cytometry , haematopoiesis , stem cell , immunology , medicine , andrology , chemistry , microbiology and biotechnology , biology , in vitro , biochemistry , platelet
Background and Objectives:Optimal mobilization and harvest of hematopoietic progenitors are essential for peripheral blood stem cell transplantation after myeloablative high‐dose chemotherapy. Conflicting data have been published concerning the most useful, cost‐effective collection strategy which is also convenient for patients. Materials and Methods: A total of 66 leukaphereses in 20 patients were retrospectively evaluated. We assessed the predictive value of the number of white blood cells, mononuclear cells (MNCs) and CD34+ cells in peripheral blood for the yield of CD34+ cells in leukapheresis products. The concentrations of MNCs and CD34+ cells were quantified simultaneously by a flow cytometric procedure using fluorescent microparticles. Their collection efficiencies were calculated based on a newly developed formula. Results: The collected hematopoietic progenitor concentration could be predicted only by the number of peripheral blood CD34+ cells prior to apheresis (r = 0.902; p < 0.01). Furthermore, the mobilization of at least 30 CD34+ cells/μl peripheral blood was a good predictor that a single leukapheresis would yield a minimum of 2.0 × 10 6 CD34+ cells/kg body weight. The collection efficiencies calculated by the new formula were 55.2±10.7% and 57.7±11.2% for MNCs and CD34+ cells, respectively. Conclusion: The precise quantification of MNCs and CD34+ cells by a direct flow cytometric assay, as well as the new formula to determine the collection efficiencies, has an impact on optimizing high‐quality stem cell products.