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D Va Category Phenotype and Genotype in Japanese Families
Author(s) -
Legler T.J.,
Wiemann V.,
Ohto H.,
Matuda I.,
Obara T.,
Uchikawa M.,
Köhler M.
Publication year - 2000
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2000.7830194.x
Subject(s) - phenotype , genetics , exon , allele , genotype , gene , genotype phenotype distinction , biology , restriction enzyme
Background and Objectives:The genetic background of the D Va category phenotype has been described in two Caucasian individuals. We were interested in the RHD sequence of 7 Japanese D Va individuals and their families. Materials and Methods: With SSP‐PCR we tested exons 4, 5 and 7 of the RHD gene and used restriction enzymes for testing nucelotide associated with the D Va phenotype. Results: A single RHD G667 C697 allele was present in 5 individuals with D Va category phenotype, and in 2 individuals we found a D‐CE‐D hybrid gene (exon 5 had been replaced). The D Va Ce gene complex was found in all families. Conclusion: The changes of the RHD gene described in European D Va individuals were also observed in Japanese families.