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Preparation of Leukocyte‐Poor Platelet Concentrates via a Short, Hard Spin of a Pool of Buffy Coats
Author(s) -
Delden C.J.,
Faber R.D.,
Wit H.J.C.,
Sibinga C. Th. Smit
Publication year - 2000
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2000.7830164.x
Subject(s) - platelet , buffy coat , centrifugation , chromatography , chemistry , immunology , biology
Background and Objectives:A new method for the preparation of leukocyte‐poor platelet concentrates was developed, based on a short, hard spin of a pool of 5 buffy coats (BCs) combined with automated collection of the platelets. Materials and Methods: The characteristics of platelet concentrates (PCs) were studied as a function of the total g force applied to a pool of 5 BCs. Pools of BCs were centrifuged for 1 min with a total g force ranging from about 3,300 to 5,000 gmin (n = 7–9 per applied g force). Deceleration took place without the means of a brake. The total centrifugation time was about 11 min. The platelet‐rich plasma (PRP) fraction above the cell layer was separated by an automated component preparation device. Results: A short, hard spin with a total g force of between 3,400 and 4,600 gmin resulted in PCs that contained on average more than 290×10 9 platelets and less than 5×10 6 leukocytes without the use of a leukocyte filter, provided that the transfer of PRP was electronically checked and terminated. The cell concentrations in the PCs are a function of the total g force. Both the platelet and leukocyte levels in the concentrate decreased with an increase in the total g force applied to the pool. Conclusion: The preparation of PCs via a short hard, spin of BCs, combined with automated collection of the PRP molecular may be an alternative method for the preparation of leukocyte‐poor PCs.