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Extracorporeal Plateletpheresis Induces the Interaction of Activated Platelets with White Blood Cells
Author(s) -
Gutensohn K.,
Alisch A.,
Krueger W.,
Kroeger N.,
Kuehnl P.
Publication year - 2000
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.2000.7820101.x
Subject(s) - platelet , plateletpheresis , apheresis , cd63 , flow cytometry , platelet activation , p selectin , immunology , cd19 , white blood cell , chemistry , monocyte , medicine , andrology , microbiology and biotechnology , biology , biochemistry , microrna , microvesicles , gene
Background and Objectives:In this study we investigated whether platelet activation during apheresis results in the binding of platelets to white blood cells. Material and Methods: Analysis of platelet–leukocyte interaction was performed using multiparameter, three–color flow cytometry. Results: Over the duration of the procedure, there was an increase in the surface expression of CD62p (P–selectin) and CD63 (p<0.05), and also in the binding of platelets to monocytes (p<0.05), neutrophilic granulocytes (p<0.05) and to CD3+ cells (initially to a low degree; p<0.05). Platelet binding to CD19+ cells did not change significantly. Conclusion: This study demonstrates that platelets become activated during apheresis and that following this process, interaction with monocytes and neutrophilic granulocytes occurs.