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Preparation of Leukodepleted Platelet Concentrates from Pooled Buffy Coats: Prestorage Filtration with Autostop™BC
Author(s) -
Pietersz R.N.I.,
Meer P.F.,
Steneker I.,
Hinloopen B.,
Dekker W.J.A.,
Zanten A.P.,
Reesink H.W.
Publication year - 1999
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1999.7640231.x
Subject(s) - leukoreduction , hemocytometer , platelet , blood product , buffy coat , filtration (mathematics) , whole blood , centrifugation , chemistry , hematocrit , chromatography , andrology , medicine , immunology , surgery , biochemistry , statistics , mathematics
Background and Objectives:Our requirements for leukocyte‐depleted platelet concentrates (LD‐PC) for an adult patient are: platelets >240×10 9 , leukocytes <5×10 6 , volume of 150–400 ml; and at the end of storage a pH between 6.8 and 7.4 and presence of the swirling effect. Our aim was to develop a standardized, semiautomated method for the production of LD‐PC, by pooling of buffy coats (BC), and prestorage leukoreduction by filtration. Materials and Methods: Whole blood was collected in Top and Bottom systems, and separated automatically with the Compomat™ G3 equipment into a red cell concentrate, a plasma and a BC. Subsequently, a pool of 5 BC was made, and 200 g plasma from one of the donors was added. Then, after soft spin centrifugation, the platelet rich plasma was leukocyte depleted by filtration using the Autostop™ BC filter, and stored in a 1,000 ml polyolefin platelet storage bag. Results : BC (n = 60) had a volume of 51±2 ml (mean ± SD) with a hematocrit of 0.44±0.03 I/I and contained 80±5% of the platelets and 74±12% of the leukocytes of the whole blood. Routinely prepared LD‐PC (n = 15,037) contained a median of 341×10 9 platelets (range 49‐599×10 9 ), with only 104/15,037 (0.7%) containing fewer than 240×10 9 platelets; the median volume was 263 ml (range 134‐373 ml). In 118/917 (13%) LD‐PC leukocytes were observed in the Nageotte hemocytometer, but only twice exceeding 1×10 6 leukocytes per unit, and none exceeding 5×10 6 (median <0.6×10 6 ; range <0.6‐1.41×10 6 ). Storage experiments of the LD‐PC (n = 12) revealed adequate oxygenation and maintenance of pH and swirling effect up to 9 days. Conclusions: This method warrants with 99% confidence that LD‐PC contain more than 240×10 9 platelets; with 97.5% confidence that 100% of the LD‐PC contain <5×10 6 leukocytes, and with 95% confidence that more than 99% of the LD‐PC contain fewer than 1×10 6 leukocytes; these LD‐PC can be stored satisfactorily for up to 9 days.