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A Newly Identified Nonsecretor Allele of the Human Histo‐Blood Group α(1,2) Fucosyltransferase Gene (FUT2)
Author(s) -
Yu LungChin,
Lee HuiLin,
Chu ChenChung,
Broadberry Richard E.,
Lin Marie
Publication year - 1999
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1999.7620115.x
Subject(s) - allele , fucosyltransferase , genotype , genetics , biology , gene , restriction fragment length polymorphism , microbiology and biotechnology , phenotype , restriction enzyme , coding region , polymerase chain reaction
Background and Objectives:The human Secre α(1,2) fucosyltransferase gene determines the ABH secretor status and influences the Lewis phenotype of an individual. Studies were carried out on the Lewis 〈a+b‐〉 nonsecretors of different groups indigenous to Taiwan to demonstrate their se genotypes. Meth: The Lewis phenotype of the blood samples was determined by a microplate method. The se genotypes of the individuals with the Lewis 〈a+b‐〉 phenotype were analyzed by a polymerase chain reaction restriction fragment length polymorphism (PCR‐RFLP) method designed for the se alleles reported previously. PCR and cloning techniques were used to determine the coding sequence of the novel se gene. Results: A new se allele, se 685 , with a three‐nucleotide deletion of GTGGT to GT in the coding region of nucleotides 685 through 689 was identified in a Le 〈a+b‐〉 nonsecretor from the Ami tribe indigenous to Taiwan. The deletion predicts the loss of the amino acid Val 230 in the corresponding secretor enzyme's C‐terminal segment. The distribution of the se 685 allele in the Ami tribe was further verified by PCR‐RFLP analysis. Conclusion: The se gene exhibits heterogeneity with some se alleles being common but others displaying a unique distribution in different ethnic populations. The newly identified se 685 allele seems to exist only in the Ami tribe indigenous to Taiwan.