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Efficiency of Leukocyte Removal by Filters Made of Superfine Glass Fiber Membranes
Author(s) -
Yuanguo Zou,
Qijun Sun,
Aihua Li,
Fangfang Yao,
Zhengfang Hu,
Zhen Li,
Wenrong Ma
Publication year - 1999
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1999.7610022.x
Subject(s) - membrane , glass fiber , fiber , materials science , filtration (mathematics) , chromatography , chemistry , composite material , chemical engineering , biochemistry , statistics , mathematics , engineering
Background and Objectives:To demonstrate the application of leukocyte removal filters made of a new type of filter material – superfine glass fiber – for depleting leukocytes in SAGM red cell suspensions and preventing nonhemolytic transfusion reactions. Materials and Methods: The extent of leukocyte depletion and red cell recovery was based on cell counts. Trace leukocytes were counted in a 50‐μl Nageotte counting chamber or by using a flow cytometer. The chemical stability of the glass fiber membranes was studies by plasma emission spectrometer and by measuring the ion content and weighing nonvolatile matter in water extract. The structural stability of the glass fiber membranes was studied by a micropore‐filter membrane method. Results: Leukocyte removal filters made of superfine glass fiber membranes removed more than 99.0% of leukocytes in SAGM red cell suspensions prepared from 400 ml whole blood. Red cell recovery exceeded 90%, and the total number of residual leukocytes was less than 5 × 10 6 . A water extract of the glass fiber membranes contained only traces of Si 4+ and Ca 2+ and less than 2 mg/100 ml of nonvolatile matter. No broken or loose fibers were found in the filters. Scanning electron microscopy showed that the web structure of the glass fiber membranes was instrumental in trapping and holding leukocytes. Conclusion: A filter made of glass fiber membranes is effective in leukocyte depletion.