Premium
Two Examples of an Inseparable Antibody That Reacts Equally Well with D W + and Rh32+ Red Blood Cells
Author(s) -
Reid Marion E.,
Sausais Laima,
Zaroulis Charles G.,
Mohandas Kala,
Coghlan Gail,
LomasFrancis Christine
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7530230.x
Subject(s) - antibody , epitope , antigen , microbiology and biotechnology , exon , phenotype , chemistry , red blood cell , nucleotide , reactivity (psychology) , gene , biology , biochemistry , genetics , medicine , pathology , alternative medicine
Background and Objectives : Anti‐D W is a rare specificity that detects an antigen on D Va red blood cells (RBCs). Some anti‐D W contain an inseparable component that cross‐reacts weakly with RBCs expressing the low‐incidence Rh antigen Rh32. RH32 is expressed by RBCs with either the R N or the DBT phenotype. Case Report : We describe here an antibody found in the serum of 2 patients that reacts equally well with RBCs possessing either D Va , R N , or DBT phenotypes. The reactivity for D W and Rh32 antigens could not be separated by adsorption onto and elution from D W +Rh32‐or from D W ‐Rh32+RBCs. Conclusions : We suggest that amino acids encoded by nucleotides at the junction of exon 4 of RHD to exon 5 of RHCE may induce a conformation that is recognized by these equally reactive inseparable antibodies. Until such time that the epitope recognized by these antibodies is defined, we recommend use of the descriptive name anti‐D W /Rh 32.