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Platelets Stored in a New‐Generation Container
Author(s) -
WildtEggen J.,
Schrijver J. G.,
Smid W. M.,
Joie M.,
Bollinne V.,
Bins M.
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7530218.x
Subject(s) - platelet , buffy coat , chemistry , blood product , thrombopoiesis , platelet concentrate , zoology , chromatography , andrology , surgery , medicine , biology , progenitor cell , stem cell , megakaryocyte , genetics
Background and Objectives : In order to preserve platelet concentrates (PCs) with high yields, a new polyolefin platelet storage container (PL 2410, 1.3L, Baxter, La Châtre, France) with increased gas permeability in combination with a larger surface area has been developed. The storage capacity was studied with platelets in plasma and platelets additive solution. Materials and Methods : Platelet concentrate pools (PCs) of different yields suspended in either plasma (PCs‐PL; n = 30) or PAS II (PCs‐PAS; n = 37) were prepared. For preparation of PCs with a low, intermediate and high number of platelets 3, 5 and 6 buffy coat (BCs) were pooled with different volumes of plasma and 5 and 6 BCs were pooled with different volumes of PAS II, in order to obtain PCs of equal volumes comparable with routine conditions. All PCs were stored on a flatbed shaker at 22±2°C and evaluated on days 1, 2, 5 and 7 by determining platelet and white cell counts, pH (37°C), pO 2 , pCO 2 and swirling score. Results : Platelet yields ranged from 1.5 up to 5.5 × 10 11 platelets/U. On day 7 all PCs‐PL (n = 4) with platelet yields above 4.5×10 11 had a pH value below 6.8 (range 5.91–6.79). While 7 of 8 PCs‐PAS units with platelet yields above 4.0×10 11 /U showed a pH value below 6.8 (range 6.31–6.70). Conclusion : During 7 days of storage in a new 1.3‐liter platelet container, the pH was maintained above 6.8 in PCs in plasma with a yield between 1.5‐and 4.5×10 11 /U; when PAS II was used, the maximum platelet yield allowed for comparable pH maintenance was somewhat lower (4.0x10 11 /U).