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Erythrocytes and Proinflammatory Mediators in Wound Drainage
Author(s) -
MottlLink S.,
Russlies M.,
Klinger M.,
Seyfarth M.,
Ascherl R.,
Gradinger R.
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7530205.x
Subject(s) - histamine , proinflammatory cytokine , medicine , prostaglandin e , prostaglandin e2 , surgery , inflammation
Background and Objectives : Retransfusion of shed blood collected after operation has become popular, but recent reports of side effects led to a search for possible causes. Materials and Methods : In a randomized study of 28 patients undergoing total hip arthroplasty, shed blood was collected in Solcotrans, Orth Evac, and ordinary Redon drainage. Osmotic fragility was measured and electron‐microscopic pictures of erythrocytes from selective samples were taken. Serotonin, prostaglandin E 2 (PGE 2 ), and histamine were measured with enzymelinked immunosorbent assays. Results : Higher osmotic fragility of erythrocytes collected with Solcotrans appeared to be due to ACD which was used only with that system. Serotonin concentrations did not differ significantly. However, there was a great increase in histamine (Solcotrans 477.7, Orth‐Evac 344.0, Redon drainage 453.1 nmol/ml) and PGE 2 (Solcotrans 1,908.3, Orth‐Evac 1,225.0, Redon drainage 2,666.7 μg/ml) in shed blood compared with venous blood (histamine 9.5 nmol/l, PGE 2 4.2 μg/ml). Conclusion :Unwashed wound drainage blood collected after operation contains levels of proinflammatory mediators that can account for the reported side effects.