Premium
c‐fos and c‐jun mRNA Expression in Activated Cord and Adult Lymphocytes: An Analysis by Northern Hybridization
Author(s) -
DePalma L.,
Brown E.,
Baker R.
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7520134.x
Subject(s) - lymphocyte , northern blot , cord blood , c fos , stimulation , western blot , biology , c jun , t lymphocyte , medicine , microbiology and biotechnology , cd3 , endocrinology , messenger rna , immune system , gene expression , immunology , cd8 , gene , biochemistry , transcription factor
Background and Objectives: To further analyze the neonatal immune response to an antigenic challenge such as blood transfusion, c‐fos and c‐jun mRNA expression were analyzed in twelve in‐vitro‐stimulated normal cord blood and ten in‐vitro‐stimulated normal adult peripheral blood lymphocyte samples. Materials and Methods: Lymphocyte samples were stimulated by either the mitogen phytohemagglutinin (PHA) or the monoclonal antibody αCD3. Proliferation rate and Northern blot hybridization were employed. Results: Cord lymphocytes revealed a greater proliferation rate with PHA and αCD3 than adult lymphocytes (p=0.0081 and 0.0023, respectively). In addition, Northern blot analysis of cord and adult samples revealed similar maximal increases in c‐fos (99±15 and 126±11%, p=0.0126) and c‐jun (123±9 and 185±38%, p=0.0291) mRNA expression, respectively, as early as 15 min postαCD3 stimulation. Adult lymphocytes showed an equivalent increase in mRNA expression of c‐fos and c‐jun (140±25 and 155±31%) at 30 min post‐PHA stimulation, while cord lymphocyte maximum c‐fos and c‐jun expression (82±6 and 142±12%) occurred at 15 min post‐PHA stimulation (c‐fos, p=0.0354; c‐jun, p=0.0112). Conclusion: Although cord lymphocyte proliferation rates were significantly greater than those of adult lymphocytes following stimulation, lymphocyte activation, as analyzed by c‐fos and c‐jun mRNA expression, appears similar in both cord and adult samples. We conclude that cord lymphocyte activation exhibits an adult‐type profile.