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Characterization of a Macaque Anti‐Rh17‐Like Monoclonal Antibody
Author(s) -
Blancher A.,
Roubinet F.,
Reid M.E.,
Socha W.W.,
Bailly P.,
Bénard P.
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7510058.x
Subject(s) - monoclonal antibody , macaque , rhesus macaque , immunoprecipitation , antigen , biology , microbiology and biotechnology , virology , epitope , antibody , red blood cell , monoclonal , spleen , genetics , immunology , paleontology
In order to produce macaque monoclonal antibodies (mAbs) against human red blood cell (RBC) antigens, macaques were immunized with human and gorilla RBCs and their spleen lymphocytes were fused with man‐mouse heteromyeloma cells. One macaque‐mouse heterohybridoma produced a macaque IgGχ (Cyn2‐4D5) which agglutinated all human RBCs but not rare human variants Dc–, D––, and Rh null . Thus, Cyn2‐4D5 exhibited RH17‐like reactivity. The specificity of Cyn2‐4D5 for RHCE‐encoded polypeptides was confirmed by specific immunoprecipitation of RhcE and RhCe polypeptides from human RBCs and the absence of immunoprecipitation of the RhD polypeptides extracted from D–RBCs. This study demonstrates that it is possible to produce macaque mAbs against human RBC blood group antigens.