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Different Genotypes Causing Indiscernible Patterns of A Expression on A el Red Blood Cells as Visualized by Scanning Immunogold Electron Microscopy
Author(s) -
Hansen Torjørn,
Namork Ellen,
Olsson Martin L.,
Alan Chester M,
Heier Hans Erik
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7510047.x
Subject(s) - immunogold labelling , exon , biology , microbiology and biotechnology , gene , genetics , abo blood group system , frameshift mutation , sequence (biology) , genotype , sequence analysis , antibody
Background and Objectives: The published sequence of the weak A subgroup A el gene from Swedish individuals showed a G insertion in exon VII, causing a frameshift at ccdon 268 (the A 01 gene has 353 codons). We wished to sequence exons VI and VII of two Norwegian A el individuals and compare the expression of A substance on RBC from different A el individuals. Materials and Methods: Exon VI and VII were amplified by PCR, cloned in M13 and sequenced. A structure expression on A el RBC was studied by the immunogold technique. Results: In contrast to the Swedish A el individuals, the two Norwegians had consensus A 01 sequences in exon VI and VII. However, the patterns of A expression were indiscernible from the Swedish cases as visualized by immunogold labeling in SEM. In both cases, a few (1–2%) RBC were very strongly labeled, some were weakly labeled and the majority (95%) were unlabeled. Conclusion: Although some A el individuals have an inserted nucleotide in exon VII of the ABO gene, others have consensus A 01 sequence in exon VI and VII. However, we could not find any differences in phenotype by immunogold labeling in SEM.