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Histamine Synthesis by White Cells during Storage of Platelet Concentrates
Author(s) -
Muylle L.,
Beert J. F.,
Mertens G.,
Bult H.
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7430193.x
Subject(s) - platelet , histamine , blood preservation , chemistry , white (mutation) , medicine , immunology , andrology , biochemistry , gene
Objectives: To resolve the question whether histamine, like some cytokines, is actively synthesized during storage of platelet concentrates. Methods: We prepared conventional buffy coat platelet concentrates and stored them in the usual way at 22°C. Disodium cromoglycate was added to one series, saline to the controls. Samples were taken at intervals, to be tested for histamine and interleukin‐6 (IL‐6). Results: The plasma histamine level increased from a median of 1.02 ng/ml (range 0.11–3.27) to 12.9 ng/ml (range 4.30–32.9) whereas the total histamine content of the platelet concentrates remained unchanged during the 5‐day storage period. In contrast, the total content of IL‐6 increased rapidly. Conclusion: Histamine is not synthesized during storage of platelets, whereas IL‐6 is. The addition of disodium cromoglycate, a substance that inhibits granulocyte activation, had no effect on the release of histamine or on the total histamine content at various storage times.