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New Murine Monoclonal Antibodies Directed against Glycophorins C and D, Have Anti‐Ge2 Specificity
Author(s) -
Janvier D.,
Veaux S.,
Benbunan M.
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7420101.x
Subject(s) - epitope , monoclonal antibody , glycophorin , serology , microbiology and biotechnology , antibody , antigen , monoclonal , chemistry , biology , virology , immunology
Background and Objectives : The Gerbich blood group system comprises three high‐incidence antigens which have been localized on glycophorins C (GPC) and D (GPD) molecules. Murine monoclonal antibodies (mAbs) directed against Ge4 and Ge3 epitopes have already been obtained. We describe two murine mAbs (Z151.17 and J6) with Ge2 specificity. Materials and Methods : The mAbs were investigated by serological methods and immunoblotting, with common, Ge‐negative and enzyme‐modified RBCs. Results : Both mAbs behaved like anti‐Ge2 in serological tests, but bound to GPC and GPD on immunoblots. Z151.17 did not recognize the GPC Yus or GPC Ge variants, whereas J6 revealed a 25‐kD component from Ge:‐2,‐3,4 membranes, which is identical to the GPC Ge molecule. Conclusion : These two mAbs are directed to slightly different epitopes. The Z151.17 and J6 epitopes are common to GPC and GPD, thus distinct from the Ge2 antigen defined by human alloantibodies.