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Complement‐Binding Anti‐Jk a Not Detectable by DiaMed Gels
Author(s) -
O'Brien P.,
Hopkins L.,
McCarthy D.,
Murphy S.
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7410053.x
Subject(s) - diluent , haemolysis , chemistry , antibody , hemolysis , complement system , biochemistry , immunology , biology , nuclear chemistry
Background: An anti‐Jk a was not detectable by the DiaMed‐ID Micro Typing System but was strongly reactive when tested using other indirect antiglobulin test (IAT) methods. However, it was detectable in the DiaMed‐ID system when the recommended diluent ID‐Diluent 2, was replaced by other low ionic Strength saline (LISS) solutions. This led us to believe that the problem lay with the ID‐Diluent 2. Methods: The anti‐Jk a was investigated using various low ionic strength diluents in the IAT against both polyspecific and monospecific antiglobulin reagents. We tested the ID‐Diluent 2 for its ability to inhibit complement activation, and bind calcium. Results: The anti‐Jk a was shown to be a ‘complement‐only’ binding antibody that reacted in all IATs performed except when tested with ID‐Diluent 2. The ID‐Diluent was shown to prevent haemolysis of ABO lytic antibodies, and further testing shows that it binds calcium. Conclusion: The use of a diluent which prevents haemolysis and binds calcium in an IAT system that contains anti‐complement as well as anti‐IgG must be questioned.