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Removal of Methylene Blue from Plasma via an Adsorbent Filter
Author(s) -
AuBuchon James P.,
Pickard Constance,
Herschel Louise,
O'Connor James L.,
Lee Eric
Publication year - 1998
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1998.7410001.x
Subject(s) - methylene blue , filtration (mathematics) , chromatography , apheresis , chemistry , partial thromboplastin time , adsorption , coagulation , platelet , medicine , biochemistry , statistics , mathematics , organic chemistry , photocatalysis , catalysis
Objective: To evaluate adsorbent filtration of methylene blue (MB) and leukocytes from plasma. Methods: Plasma (750 ml) from apheresis of 10 normal subjects was split into three aliquots: control (A), filtration (B) and MB addition (to 1 μ M ), phototreatment and filtration (C). Biochemical and coagulation tests were performed; units A and B were reinfused. Results: Filtration reduced MB to undetectable (<0.05 μ M ) levels and leukocytes by 3 log 10. Biochemical analytes were unchanged. The partial thromboplastin time was prolonged with MB addition (11±13%) or filtration (26±12%, p < 0.05), but the effects were not additive. Autologous transfusion was well tolerated. Conclusion: Adsorbent filtration can reduce residual MB to undetectable levels and yields a component suitable for transfusion.