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Application of the Gel Test in IgG Subclassing – A Comparison of Two Agglutination Assays
Author(s) -
Palfi Miodrag,
Hildén JanOlof
Publication year - 1997
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1997.7220114.x
Subject(s) - polyclonal antibodies , agglutination (biology) , antibody , direct agglutination test , immunology , hemolytic disease of the newborn (abo) , microbiology and biotechnology , chromatography , chemistry , medicine , biology , serology , pregnancy , fetus , genetics
Background and objectives: IgG subclasses of anti‐D seem to play some role in hemolytic disease of the newborn, but there has been disagreement as to its exact nature. The aim of our study was to evaluate a new technique for IgG subclassing of anti‐D and to compare it to an established test. Materials and methods: In 31 cases of RhD immunization, we have compared two simple agglutinating assays for subclassing anti‐D: an established V‐well microtiter tray assay and a new gel test assay. Polyclonal rabbit anti‐IgG agglutinating antibodies were tested against sensitized D‐positive red blood cells by both assays. Results: The anti‐D concentration in tested sera varied between 0.1 and 81 μg/ml (0.5–405 IU/ml). Both assays were simple to perform and the results correlated well. Conclusions: The gel test had two advantages: quick performance and easy interpretation.