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Leukocyte Filtration of Single‐Donor Platelet Units through an Integrated Filter System for the Fresenius AS 104 Blood Cell Separator
Author(s) -
Moog R.,
Müller N.,
Köbke A. C. H.,
Kalb R.
Publication year - 1997
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1997.7220107.x
Subject(s) - platelet , apheresis , plateletpheresis , blood product , filtration (mathematics) , contamination , blood cell , white blood cell , leukoreduction , chromatography , chemistry , medicine , immunology , surgery , biology , mathematics , ecology , statistics
Background and objectives: White cell depletion of platelet concentrates is widely applied to reduce the incidence of transfusion complications. We investigated a new plateletpheresis kit with an integrated filter for leukocyte depletion of platelets. Materials and methods: Parameters of product quality were investigated before and after in‐line filtration of 70 single‐donor platelet (SDP) units obtained by apheresis. We studied three different filter batches manufactured for the Fresenius AS 104 blood cell separator, and evaluated different softwares and interface positions with respect to platelet (PLT) yield and white blood cell (WBC) contamination. Results: PLT yields before and after filtration was not statistically different in the protocols studied. WBC contamination after WBC depletion was in the range of 10 4 /SDP unit. Glucose, lactate dehydrogenase, and the morphology score were not statistically different before and after filtration. Conclusions: In‐line WBC filtration of SDP units sufficiently reduced WBC below 5×10 6 /unit. The study allowed definition of the filter composition and apheresis parameters to optimize PLT yield and minimize WBC contamination.