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Evaluation of a New Prestorage Leukoreduction Filter for Red Blood Cell Units
Author(s) -
AuBuchon J. P.,
Elfath M. D.,
Popovsky M. A.,
Stromberg R. R.,
Pickard C.,
Herschel L.,
Whitley P.,
McNeil D.,
Arnold N.,
O'Connor J. L.
Publication year - 1997
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1997.7220101.x
Subject(s) - leukoreduction , hemolysis , in vivo , red cell , filtration (mathematics) , red blood cell , leukapheresis , biology , andrology , medicine , immunology , blood transfusion , microbiology and biotechnology , mathematics , statistics , genetics , stem cell , cd34
Background and objectives: Prestorage leukoreduction offers a variety of potential benefits and is becoming more commonly practiced. The LeukoNet™ prestorage leukoreduction filtration system is intended for leukoreduction of red blood cells and uses a vent to allow automatic drainage of red cells from the filter. Materials and methods: We studied the functional characteristics and the in‐vivo and in‐vitro properties of leukoreduced AS‐1 Red Blood Cells prepared with this new system. Units of AS‐1 Red Blood Cells were filtered at 4°C through the LeukoNet™ filter 24–48 h after collection and stored under usual conditions for 42 days. Residual leukocytes were enumerated using a Nageotte chamber or with a polymerase chain reaction (PCR) technique. In the clinical trial (phase one), 21 donors had units stored with and without leukoreduction for 42 days; biochemical assays were done before and after storage, and 51 Cr/ 99 mTc red cell recovery studies at the end of the storage period. Results: Leukocyte content after filtration was 3.2±2.6×10 4 /unit (n = 21), and all units had <1×10 5 leukocytes (median: 3.8×10 4 ). In‐vivo paired studies showed no difference in 24‐hour recovery (control: 82.1±5.8%; test: 82.9±6.0%). Hemolysis was halved with leukoreduction (0.59±0.30 vs. 0.29±0.11%; p<0.05), and glucose consumption was reduced by 5% compared to control units (p = <0.05). Other biochemical parameters showed no differences. In the practical trial (phase two), filtration time was 41±23 min. With a residual leukocyte content of 6.6±4.9×10 4 /unit and 14±3% red cell loss (n = 84). Six additional units underwent leukocyte enumeration by PCR and had 2.6±1.1×10 4 residual leukocytes. Conclusions: Under the conditions studied, the LeukoNet™ leukoreduction filtration system produces about 4–5 log 10 leukocyte content reduction.