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A Monolayer Coagglutination Microplate Technique for Typing Red Blood Cells
Author(s) -
Llopis F.,
CarbonellUberos F.,
Planelles MD.,
Montero M.,
Puig N.,
Atienza T.,
Alba E.,
Montoro J.A.
Publication year - 1997
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1997.00026.x
Subject(s) - abo blood group system , hemagglutination , monolayer , agglutination (biology) , red cell , red blood cell , typing , rh blood group system , chemistry , chromatography , microbiology and biotechnology , immunology , medicine , biology , antibody , biochemistry
Background and objectives: Serologic agglutination tests have the disadvantage of lack of an objective endpoint that can be easily read and quantitated. We have developed a new method for ABO and Rh typing based on producing a red blood cell (RBC) monolayer on microplates and the mixed hemagglutination reaction. Materials and methods: We have employed a new red cell fixation buffer to prepare the RBC monolayer. As the technique for grouping, we used a mixed agglutination reaction between the RBC monolayer and the RBCs to be typed. Results: Compared with an automated hemagglutination system in 34,519 samples, the method is shown to be more sensitive, free of false positive reactions, and cost‐effective. Conclusions: The microplate coagglutination method is accurate and lower in cost than conventional methods.