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Rapid Typing for Human Platelet Antigen Systems ‐1, ‐2, ‐3 and ‐5 by PCR Amplification with Sequence‐Specific Primers
Author(s) -
Klüter Harald,
Fehlau Kirstin,
Panzer Simon
Publication year - 1996
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1996.7120121.x
Subject(s) - typing , polymerase chain reaction , sequence (biology) , antigen , biology , microbiology and biotechnology , computational biology , virology , genetics , gene
Typing for human platelet antigens (HPA) is useful in a variety of clinical situations. We developed a method for genotyping for HPA‐1, ‐2, ‐3 and ‐5 by means of the PCR amplification with sequence‐specific primers (PCR‐SSP) technique. Primer sets were designed to allow PCR amplification for all systems using the same assay conditions. Specificity and sensitivity of the method were assessed in a blind quality control study (n = 112). In 111 cases, results obtained by PCR‐SSP were identical as compared with PCR‐restriction fragment length polymorphism technique. One discrepancy was found to be due to a typing error in the data sheet. The results of the PCR‐SSP technique were available within 3 h. We conclude that genotyping based on PCR‐SSP enables rapid typing for HPA systems, which makes this technique feasible in most clinical settings where urgent HPA typing is required.