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Comparison of Solid‐Phase Antibody Screening Tests with Pooled Red Cells in Blood Donors
Author(s) -
Schrem A.,
Flegel W.A.
Publication year - 1996
Publication title -
vox sanguinis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.68
H-Index - 83
eISSN - 1423-0410
pISSN - 0042-9007
DOI - 10.1046/j.1423-0410.1996.7110037.x
Subject(s) - antibody , titer , medicine , immunology , antibody titer
Solid‐phase systems are very sensitive for detection of erythrocyte alloantibodies in serum and suitable for large scale donor screening. Serum samples of 10,008 blood donors were screened by three solid‐phase tests (Capture R Ready Screen, Solidscreen II, and Solidscreen II‐Donor) with pooled red cells derived from two donors. The prevalence of antierythrocyte IgG and IgM antibodies in donor sera was 0.56% (IgG antibodies: 0.42%). The test efficiency for IgG antibodies was 1.40 with Capture R Ready Screen (test sensitivity 97.6%, test specificity 99.39%), 1.78 with Solidscreen II (95.1, 99.88%), and 1.95 with Solidscreen II‐Donor (92.7, 99.98%). The IgG antibody titers differed significantly between all tests including a gel matrix test: Capture R > ID‐Gel > Solidscreen II > Solidscreen II‐Donor. Previously characterized antibodies that were not detected after long‐term follow‐up by any of the three solid‐phase tests had a prevalence of 0.10%. All three solid‐phase tests detected the alloantibodies, which were of higher titers and considered clinically relevant in blood components. The significant difference in antibody titers between the tests was not matched by a similar variance in the detection of donors with antibodies. Even with sensitive solid‐phase tests, many antibodies may not be detected after long‐term follow‐up.